Basics of genetic engineering and diagnostics (5 cr)
Code: 5021169-3003
General information
Enrollment
01.12.2019 - 16.01.2020
Timing
13.01.2020 - 20.03.2020
Number of ECTS credits allocated
5 op
Mode of delivery
Contact teaching
Unit
Engineering and Business
Campus
Kupittaa Campus
Teaching languages
- English
Seats
10 - 30
Degree programmes
- Degree Programme in Chemical and Materials Engineering
Teachers
- Sari Loppela-Rauha
- Mika Jokinen
- Jarno Pusa
- Jani Pelkonen
- Sari Airenne
Groups
-
PPROMS17
-
PPROMS17BioPPROMS17Bio
Objective
The participants are able to describe the essentials of diagnostics and basic concepts and methods of diagnostics and genetic engineering. The participants know the basic principles and methods of genetic engineering.
Content
- basics and basic techniques of genetic engineering
- essentials, concepts and methods of diagnostics
Materials
Suominen I., Pärssinen, R., Haajanen, K. ja Pelkonen, J. Geenitekniikka (finnish)
Brown: Gene Cloning &DNA Analysis (english)
Teaching methods
Group assignments, mid-tests (Socrative), Exam
Laboratory experiments, Lab reports
Exam schedules
Exam on week 12.
Retakes according to deparment retake schedule (24.4.20 and 29.5.20).
International connections
Flipped learning, Contact teaching
Completion alternatives
Conducting research in a research group. For further information contact Mika Jokinen.
Student workload
Teoretical part:
Lectures and exam 30 h
Group assignment 10 h
Mid-test 4 h
Self-studying 36 h
Laboratory experiments:
24 h laboratory works (3 weeks, 8 h / week)
29 h self-studying and reporting of laboratory works
Content scheduling
The student should understand the core principles of genetic engineering and diagnostic practices, and know the basic terminology and methods. The course consist of theoretical part (3 cr) and laboratory woks (2 cr).
week 4: Nucleic acids & molecular biology
week 5: Requirements of gene technology, Isolation and analysis of nucleic acids
week 6: Cloning vectors, Regulation of gene activity, expression, autoinduction
week 7: Nucleic acids modifying enzymes. Ligation., Introducing DNA into host cells, PCR
week 9: DNA sequencing, Antibodies, basics of immunology
week 10: Immuno assays
week 11: Immuno diagnostics
Laboratory works:
week 9: AMPLIFICATION OF EGFP GENE BY PCR
week 9: RESTRICTION ENZYMES AND AGAROSE GEL ELECTROPHORESIS
week 10: GEL EXTRACTION OF THE DNA FROM AGAROSE GEL ELECTROPHORESIS
week 10: CLONING OF PCR AMPLIFIED DNA FRAGMENT AND TRANSFORMATION
week 11: CHARACTERIZATION OF TRANSFORMANTS
Evaluation scale
H-5