Study Guide

Objective

The participants are able to describe the essentials of diagnostics and basic concepts and methods of diagnostics and genetic engineering. The participants know the basic principles and methods of genetic engineering.

Content

- basics and basic techniques of genetic engineering

- essentials, concepts and methods of diagnostics

Materials

Suominen I., Pärssinen, R., Haajanen, K. ja Pelkonen, J. Geenitekniikka (finnish)
Brown: Gene Cloning & DNA Analysis (english)

Teaching methods

Group and individual assignments
Laboratory experiments, Lab reports

Exam schedules

will be updated beginning of the course

International connections

Flipped learning, Contact teaching

Completion alternatives

Conducting research in a research group. For further information contact Mika Jokinen (mika.jokinen@turkuamk.fi).

Student workload

Theoretical part:
Lectures, presentations, assignments and self-studying (approx. 78 h)

Laboratory experiments:
24 h laboratory works (3 weeks, 8 h / week)
29 h self-studying and reporting of laboratory works

Content scheduling

The student should understand the essential principles of genetic engineering and diagnostic practices, and know the basic terminology and methods. The course consist of theoretical part (3 cr) and laboratory woks (2 cr).

Spesific topics include:

-Nucleic acids & molecular biology
-Requirements of gene technology, Isolation and analysis of nucleic acids
-Cloning vectors, Regulation of gene activity, expression, autoinduction
-Nucleic acids modifying enzymes. Ligation., Introducing DNA into host cells, PCR
-DNA sequencing, Antibodies, basics of immunology
-Immuno assays
-Immuno diagnostics

Laboratory works:
week 7: AMPLIFICATION OF EGFP GENE BY PCR
week 7: RESTRICTION ENZYMES AND AGAROSE GEL ELECTROPHORESIS
week 9: GEL EXTRACTION OF THE DNA FROM AGAROSE GEL ELECTROPHORESIS
week 9: CLONING OF PCR AMPLIFIED DNA FRAGMENT AND TRANSFORMATION
week 10: CHARACTERIZATION OF TRANSFORMANTS

Evaluation scale

H-5

Assessment methods and criteria

Theory part (60%) and Laboratory part (40%).