Basics of genetic engineering and diagnostics (5 cr)
Code: 5021169-3006
General information
Enrollment
10.12.2022 - 15.01.2023
Timing
09.01.2023 - 10.03.2023
Number of ECTS credits allocated
5 op
Mode of delivery
Contact teaching
Unit
Engineering and Business
Campus
Kupittaa Campus
Teaching languages
- English
Seats
0 - 20
Degree programmes
- Degree Programme in Biotechnology and Chemical Engineering
Teachers
- Jani Pelkonen
Groups
-
PBIOKES20
-
PBIOKES20bioPBIOKES20bio
Objective
The participants are able to describe the essentials of diagnostics and basic concepts and methods of diagnostics and genetic engineering. The participants know the basic principles and methods of genetic engineering.
Content
- basics and basic techniques of genetic engineering
- essentials, concepts and methods of diagnostics
Materials
Suominen I., Pärssinen, R., Haajanen, K. ja Pelkonen, J. Geenitekniikka (finnish)
Brown: Gene Cloning & DNA Analysis (english)
Lecture notes on Its-platform
Teaching methods
Group and individual assignments
Laboratory experiments, Lab reports
Exam schedules
will be updated beginning of the course
International connections
Flipped learning, Contact teaching
Completion alternatives
Conducting research in a research group. For further information contact Jani Pelkonen (jani.pelkonen@turkuamk.fi).
Student workload
Theoretical part:
Lectures, presentations, assignments and self-studying (approx. 78 h)
Laboratory experiments:
24 h laboratory works (3 weeks, 8 h / week)
29 h self-studying and reporting of laboratory works
Content scheduling
The student should understand the essential principles of genetic engineering and diagnostic practices, and know the basic terminology and methods. The course consist of theoretical part (3 cr) and laboratory woks (2 cr).
Spesific topics include:
-Nucleic acids & molecular biology
-Requirements of gene technology, Isolation and analysis of nucleic acids
-Cloning vectors, Regulation of gene activity, expression, autoinduction
-Nucleic acids modifying enzymes. Ligation., Introducing DNA into host cells, PCR
-DNA sequencing, Antibodies, basics of immunology
-Immuno assays
-Immuno diagnostics
Laboratory works:
* AMPLIFICATION OF EGFP GENE BY PCR
* RESTRICTION ENZYMES AND AGAROSE GEL ELECTROPHORESIS
* GEL EXTRACTION OF THE DNA FROM AGAROSE GEL ELECTROPHORESIS
* CLONING OF PCR AMPLIFIED DNA FRAGMENT AND TRANSFORMATION
* CHARACTERIZATION OF TRANSFORMANTS
Evaluation scale
H-5
Assessment methods and criteria
Theory part (60%) and Laboratory part (40%).