Methods in biotechnology (5 cr)

Evaluation scale

H-5

Content scheduling

Methods in Biotechnology will be implemented as a conventional lab implementation consisting of three larger work topics.

PRELIMINARY CONTENT:
1) Bioreactor cultivation of E. coli and production of mEGFP protein.
2) Downstream and purification (heat treatment + affinity chromatography) of mEGFP including gel and total protein analysis.
3) Production of hydrogel ceramic composite, 3D printed implant, crosslinking with different times and rheological analyses of material properties.

The reporting consists of one video report and one conventional laboratory report both made by group. Deadline for video reporting is two weeks from the end of downstream labwork. Hydrogel 3D printing and rheology labwork report (conventional) deadline is 15.1.2025.

Objective

The participants are able to prepare biomaterials for tissue engineering and to describe the specific features of the preparation process. The participants are able to manufacture typical molecules for diagnostics or other industrially important molecules by bioprocesses. The participants are able to use the most important analytical methods used in biomaterials characterization, genetic engineering, diagnostics and bioprocesses

Content

- laboratory works (analytical methods and preparation) in tissue engineering, diagnostics, bioprocesses and genetic engineering

Location and time

Weeks 47-50, Lemminkäisenkatu

Materials

Most of the material will be available on Its-learning environment. Some material student need to find itself according to instructions on Its

Teaching methods

Laboratory work in groups, team-based learning in groups

Exam schedules

No exam, assessment is based on reporting, study plans, overall performance in laboratory and laboratory notebook.

International connections

Hands on practical training.
Sustainable development aspect is taken into consideration for example in laboratory work scaling, reagent consumption and waste management.

Completion alternatives

2-4 students may conduct the course by working in the the research group (Biomaterials and Diagnostics). This type of the implementation has to be agreed separate with the instructors (Jani Pelkonen and Kari Haajanen).

Student workload

Approximation:
Preparing and self study for labworks: 28 h
* pretasks for labworks and self study
Laboratory works: 72 h
* three topics, one topic per week and three lab days (8 h) per week (-> 72 h)
Reporting: 30
* Compiling the laboratory report